Last edited by Nikorn
Sunday, May 17, 2020 | History

3 edition of Practical Fluorescence Microscopy in Mammalian Cells found in the catalog.

Practical Fluorescence Microscopy in Mammalian Cells

Alexei Mikhailov

Practical Fluorescence Microscopy in Mammalian Cells

Protein Localization and Function

by Alexei Mikhailov

  • 262 Want to read
  • 33 Currently reading

Published by Humana Press .
Written in English

    Subjects:
  • Marsupials & monotremes,
  • Microscopy,
  • Microscopes & Microscopy,
  • Science,
  • Science/Mathematics

  • The Physical Object
    FormatHardcover
    Number of Pages350
    ID Numbers
    Open LibraryOL12325569M
    ISBN 101588290611
    ISBN 109781588290618
    OCLC/WorldCa52359661

    mammalian cells; this has been applied profitably to the study of cytochemical aspects of virus cytopathology (Anderson, Armstrong, and Niven, ). For routine detection of mycobacteria in smears or tissue sections, fluorescence microscopy Cited by: 6.   In addition to the volume of reagent, the optimal amount of protein to be used per well needs to be determined. The efficiency of protein delivery in HeLa cells was analysed by fluorescence microscopy (data not shown) and subsequently quantified by FACS analysis (Figs. 3 a, b). A non-fluorescent protein (1 μg), Bovine Serum Albumin Cited by:

    Illustrated in Figure 2 are summed widefield total internal reflection microscopy (TIRFM) and PALM single-molecule images of optical highlighter fluorescent proteins fused to targeting peptides or proteins that were localized within fixed mammalian cells. The fluorescence lifetime of NN-AP in yeast cells was temperature-dependent ( ns at 15 °C and ns at 35 °C), and the evaluated temperature resolution was – °C within this temperature range. In addition, NN-AP and NN/NI-AP readily entered and functioned within mammalian by:

    Mammalian cell viability assays Cell membranes in damaged and dying cells have increased permeability and allow the influx of dyes. Combination assays measure multiple parameters to provide more context for determining the status of both live and dead cells. Fluorescence Microscopy Selected Literature References. The field of fluorescence microscopy is experiencing a renaissance with the introduction of new techniques such as confocal, multiphoton, .


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Practical Fluorescence Microscopy in Mammalian Cells by Alexei Mikhailov Download PDF EPUB FB2

Buy Practical Fluorescence Microscopy in Mammalian Cells: Protein Localization and Function on FREE SHIPPING on qualified orders Practical Fluorescence Microscopy in Mammalian Cells: Protein Localization and Function: Alexei (EDT) Mikhailov Alexei Mikhailov: : Books.

Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy methods as applied to biological and biomedical science.

With contributions selected for clarity, utility, and reproducibility, the work provides practical tools. Fluorescence Microscopy: From Principles to Biological Applications 2nd Edition by Ulrich Kubitscheck (Editor) ISBN ISBN Why is ISBN important.

ISBN. This bar-code number lets you verify that you're getting exactly the right version or edition of a book Format: Hardcover.

Practical Fluorescence Microscopy in Mammalian Cells book Manual for Fluorescence Microscopy Techniques: The manuals focus lies on the core F-techniques FRET, FLIM, FCS, and FRAP, but recognizes recent developments that lead to many interesting variants currently in use, such as fluorescence.

Fluorescence nanoscopy uniquely combines minimally invasive optical access to the internal nanoscale structure and dynamics of cells and tissues with molecular detection by: Mechanism and Xia et al.

-Practical three color live cell imaging by widefield microscopy 68 applications. J Biol Chem ; (31)   Microscopy Microscope Live cell Microinjection Imaging Confocal Fluorescence Phototoxicity This is a preview of subscription content, log in to check access.

Springer Nature is Cited by: 9. An excitation and emission filter set ( nm and nm) was suitable for green fluorescent protein and yellow fluorescent protein imaging of cells, and > μM luciferin was acceptable.

Description The second edition of Live Cell Imaging: A Laboratory Manual expands upon and extends the collection of established and evolving methods for studying dynamic changes in living cells and organisms presented in the well-known first edition.

There are 16 new chapters and the 21 updated chapters in this new edition. They include advances in atomic force microscopy. The other chapters in this book give the reader an in-depth description of every important aspect of biological confocal microscopy that we could think of.

P.,CCD cameras for fluorescence imaging of living cells, In: Live Cell Imaging: A Laboratory Manual (R.D.

Goldman and D.L. Spector, edsPractical confocal microscopy Cited by: Live cell fluorescence microscopy using fluorescent protein tags derived from jellyfish and coral species has been a successful tool to image proteins and dynamics in many species.

Multi-colored aequorea fluorescent protein (AFP) derivatives allow investigators to observe multiple proteins simultaneously, but overlapping spectral properties sometimes require the use of sophisticated and expensive by: 6.

The physics and biology of fluorescence microscopy in the life porary Physics (). Brown, C. Fluorescence microscopy: avoiding the pitfalls. Journal of Cell Science (). Coling, D. and Kachar, B. Principles and Application of Fluorescence Microscopy. Schematic of a fluorescence microscope.

The majority of fluorescence microscopes, especially those used in the life sciences, are of the epifluorescence design shown in the diagram. Light of the excitation wavelength illuminates the specimen through the objective lens.

The fluorescence. Fluorescence Microscopy is the revised, updated and expanded second edition of a best-selling introductory text by professor Herman on the use of fluorescence microscopy in cell biology and related arenas.

The book covers the fundamental principles of fluorescence, configuration and operation of the microscope, as well as the application of these concepts to practical fluorescence microscopy.

Figure 2. Principals of confocal microscopy. In wide-field fluorescence microscopy, the final image consists of light emanating from multiple focal planes (left panel) thereby decreasing the signal-to-noise ratio in the final image. The confocal microscope Cited by: Fluorescence microscopy of live cells has become an integral part of modern cell biology.

Fluorescent protein (FP) tags, live cell dyes, and other methods to fluorescently label proteins of interest provide a range of tools to investigate virtually any cellular process under the microscope.

Lecture 1 - Fluorescence Microscopy. And most cell cultures need a particular set of atmospheric conditions, for example, most cell growth medias for mammalian cell cultures rely on a carbonate.

Optical voltage imaging in neurons: moving from technology development to practical tool although lack of function in mammalian cells turned out to be a major setback in the Cited by: 1. This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy.

A practical guide to the instrumentation and the application of this method in mammalian cell biology. Information is included on: Fluorescence and immunofluorescence, DNA analysis, Chromosomes analysis and sorting, Study of cell.

Fluorescence Microscopy Barry R Masters, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA The fluorescence microscope (wide-field, scanning, con-focal, one-photon excitation, multiphoton excitation) is an extremely useful and ubiquitous instrument in bio-logical and medical laboratories.

The fluorescence. Cotton A cross section of cotton stained with Rhodamine B. Mammalian Cells Fluorescence double-labeling of mammalian cells. The DNA in the cell nuclei are shown in blue. .Cellular and Molecular Applications of Super-resolution Microscopy. Summary Fluorescence Microscopy: Super-Resolution and other Novel Techniques delivers a comprehensive review of current advances in fluorescence microscopy .